{"id":533,"date":"2020-01-02T02:20:42","date_gmt":"2020-01-02T02:20:42","guid":{"rendered":"https:\/\/ladomerylab.org\/?post_type=research_papers&#038;p=533"},"modified":"2023-03-04T11:08:03","modified_gmt":"2023-03-04T11:08:03","slug":"xp54-the-xenopus-homologue-of-human-rna-helicase-p54-is-an-integral-component-of-stored-mrnp-particles-in-oocytes","status":"publish","type":"research_papers","link":"https:\/\/ladomerylab.org\/research-papers\/xp54-the-xenopus-homologue-of-human-rna-helicase-p54-is-an-integral-component-of-stored-mrnp-particles-in-oocytes\/","title":{"rendered":"Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes"},"content":{"rendered":"<p>In investigating the composition of stored (maternal) mRNP particles in Xenopus oocytes, attention has focussed primarily on the phosphoproteins pp60\/56, which are Y-box proteins involved in a general packaging of mRNA, We now identify a third, abundant, integral component of stored mRNP particles, Xp54, which belongs to the family of DEAD-box RNA helicases, Xp54 was first detected by its ability to photocrosslink ATP Subsequent sequence analysis identifies Xp54 as a member of a helicase subfamily which includes: human p54, encoded at a chromosomal breakpoint in the B-cell lymphoma cellline, RC-kappa 8;Drosophila ME31 B, encoded by amaternally-expressed gene, and Saccharomyces pombe Ste13, cloned by complementation of the sterility mutant stela Expression studies reveal that the gene encoding Xp54 is transcribed maximally at early oogenesis: no transcripts are detected in adult tissues, other than ovary, Using a monospecific antibody raised against native Xp54, its presence in mRNP particles is confirmed by immunoblotting fractions bound to oligo(dT)-cellulose and separated by rate sedimentation and buoyant density, On isolating Xp54 from mRNP particles, it is shown to possess an ATP-dependent RNA helicase activity, Possible functions of Xp54 are discussed in relation to the assembly and utilization of mRNP particles.<\/p>\n","protected":false},"template":"","display_control":[],"_links":{"self":[{"href":"https:\/\/ladomerylab.org\/wp-json\/wp\/v2\/research_papers\/533"}],"collection":[{"href":"https:\/\/ladomerylab.org\/wp-json\/wp\/v2\/research_papers"}],"about":[{"href":"https:\/\/ladomerylab.org\/wp-json\/wp\/v2\/types\/research_papers"}],"wp:attachment":[{"href":"https:\/\/ladomerylab.org\/wp-json\/wp\/v2\/media?parent=533"}],"wp:term":[{"taxonomy":"display_control","embeddable":true,"href":"https:\/\/ladomerylab.org\/wp-json\/wp\/v2\/display_control?post=533"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}