{"id":689,"date":"2022-09-04T12:18:54","date_gmt":"2022-09-04T12:18:54","guid":{"rendered":"https:\/\/ladomerylab.org\/?post_type=research_papers&#038;p=689"},"modified":"2023-03-10T12:44:22","modified_gmt":"2023-03-10T12:44:22","slug":"targeting-the-erg-oncogene-with-splice-switching-oligonucleotides-as-a-novel-therapeutic-strategy-in-prostate-cancer","status":"publish","type":"research_papers","link":"https:\/\/ladomerylab.org\/research-papers\/targeting-the-erg-oncogene-with-splice-switching-oligonucleotides-as-a-novel-therapeutic-strategy-in-prostate-cancer\/","title":{"rendered":"Targeting the ERG oncogene with splice-switching oligonucleotides as a novel therapeutic strategy in prostate cancer"},"content":{"rendered":"<p><strong>BACKGROUND: <\/strong>The <em>ERG<\/em> oncogene, a member of the ETS family of transcription factor encoding genes, is a genetic driver of prostate cancer. It is activated through a fusion with the androgen-responsive <em>TMPRSS2 <\/em>promoter in 50% of cases. There is therefore significant interest in developing novel therapeutic agents that target <em>ERG<\/em>. We have taken an antisense approach and designed morpholino-based oligonucleotides that target <em>ERG<\/em> by inducing skipping of its constitutive exon 4.<strong>\u00a0 <\/strong><\/p>\n<p><strong>METHODS: <\/strong>We designed antisense morpholino oligonucleotides (splice-switching oligonucleotides, SSOs) that target both the 5\u00a2 and 3\u00a2 splice sites of ERG\u2019s exon 4. We tested their efficacy in terms of inducing exon 4 skipping in two ERG-positive cell lines, VCaP prostate cancer cells and MG63 osteosarcoma cells. We measured their effect on cell proliferation, migration and apoptosis. We also tested their effect on xenograft tumour growth in mice and on ERG protein expression in a human prostate cancer radical prostatectomy sample ex vivo.<\/p>\n<p><strong>RESULTS: <\/strong>In VCaP cells, both SSOs were effective at inducing exon 4 skipping, which resulted in a reduction of overall ERG protein levels up to 96 hours following a single transfection. SSO-induced ERG reduction decreased cell proliferation, cell migration and significantly increased apoptosis. We observed a concomitant reduction in protein levels for cyclin D1, c-Myc and the Wnt signalling pathway member \u03b2-catenin as well as a marker of activated Wnt signalling, p-LRP6. We tested the 3\u00a2 splice site SSO in MG63 xenografts in mice and observed a reduction in tumour growth. We also demonstrated that the 3\u00a2 splice site SSO caused a reduction in ERG expression in a patient-derived prostate tumour tissue cultured ex vivo<strong>.<\/strong><\/p>\n<p><strong>CONCLUSIONS: <\/strong>We have successfully designed and tested morpholino-based SSOs that cause a marked reduction in ERG expression, resulting in decreased cell proliferation, a reduced migratory phenotype and increased apoptosis. Our initial tests on mouse xenografts and a human prostate cancer radical prostatectomy specimen indicate that SSOs can be effective for oncogene targeting in vivo. As such, this study encourages further in vivo therapeutic studies using SSOs targeting the <em>ERG<\/em> oncogene.<\/p>\n","protected":false},"template":"","display_control":[],"_links":{"self":[{"href":"https:\/\/ladomerylab.org\/wp-json\/wp\/v2\/research_papers\/689"}],"collection":[{"href":"https:\/\/ladomerylab.org\/wp-json\/wp\/v2\/research_papers"}],"about":[{"href":"https:\/\/ladomerylab.org\/wp-json\/wp\/v2\/types\/research_papers"}],"wp:attachment":[{"href":"https:\/\/ladomerylab.org\/wp-json\/wp\/v2\/media?parent=689"}],"wp:term":[{"taxonomy":"display_control","embeddable":true,"href":"https:\/\/ladomerylab.org\/wp-json\/wp\/v2\/display_control?post=689"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}